![]() ![]() ![]() Since the recognition site or sequence of base pairs is known for each restriction enzyme, we can use this to form a detailed analysis of the sequence of bases in specific regions of the DNA in which we are interested. The size of these fragments is measured in base pairs or kilobase (1000 bases) pairs. The restriction enzyme will continue to do this along the full length of the DNA molecule which will then break into fragments. Once it is located, the enzyme will attach to the DNA molecule and cut each strand of the double helix. This recognition site or sequence is generally from 4 to 6 base pairs in length. ![]() These restriction enzymes are able to scan along a length of DNA looking for a particular sequence of bases that they recognize. ![]() Special enzymes termed restriction enzymes have been discovered in many different bacteria and other single-celled organisms. ![]()
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